Isolation and characterization of mRNA from mammary gland of lactating cow.
نویسندگان
چکیده
منابع مشابه
The characterization of phosphoseryl tRNA from lactating bovine mammary gland.
BD-cellulose and RPC-5 chromatography of tRNA isolated from lactating bovine mammary gland showed the presence of four seryl-tRNA isoacceptors. The species, tRNA IV Ser, with the strongest affinity for BD-cellulose (required ethanol in the elution buffer) could be phosphorylated in the presence of serine, [gamma-32 P]-ATP, seryl-tRNA synthetase and phosphotransferase activity from the same tiss...
متن کاملThe biosynthesis of short-chain triacylglycerols by microsomal fractions from lactating-cow mammary gland.
latter were present, the amount of label in the glycerol moiety of the triacylglycerols was increased &fold and that in the diacylglycerol fraction was increased 3-fold. Fatty acids derived from glucose were found mainly in the triacylglycerols, especially in the presence of exogenous free fatty acids, although appreciable amounts of label again accumulated in the diacylglycerols. The finding t...
متن کاملFunction of arginase in lactating mammary gland.
The potential for a considerable formation of ornithine exists in lactating mammary gland because of its arginase content. Late in lactation arginase reaches an activity in the gland higher than that present in any rat tissue except liver. Occurrence of the urea cycle can be excluded since two enzymes for the further reaction of ornithine in the cycle, carbamoyl phosphate synthetase I and ornit...
متن کاملLipogenesis by acini from mammary gland of lactating rats.
Intact acini were prepared from the mammary gland of lactating rats by treatment with collagenase. The acini were obtained in high yield. The preparation offers considerable advantage over the use of tissue slices. The acini were incubated with glucose, lactate, pyruvate, and acetate labeled with 14C and tritium, as single substrates or in combination with glucose. Synthesis of fatty acids from...
متن کاملCasein kinase from the Golgi apparatus of lactating mammary gland.
A casein kinase that catalyzes the phosphorylation of dephosphorylated aSI-casein by ATP has been found in the Golgi apparatus of lactating rat mammary gland. Dephosphorylated /3and K-Caseins are also phosphorylated by this enzyme, while other milk proteins (P-lactoglobulin, a-lactalbumin, native CY,~-, fi-, and K-case& and proteins of the fat globule membrane) are phosphorylated to a limited e...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1981
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.45.177